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Synonym: 6-carboxy-4′,5′-dichloro-2′,7′-dimethoxyfluorescein N-hydroxysuccinimide ester
CAS #: 113394-23-3
Molecular Formula: C27H17Cl2NO11
Molecular Weight: 602.3
6-JOE, SE (λex/λem: 520/548nm in DMF) is the single isomer preparation of the amine-reactive dye. It has fluorescence characteristics that are red-shifted as compared to fluorescein and is one of the traditional fluorophores used in automated DNA sequencing. It is also used to label nucleotides.
1. Petersen, K., et al., Short pna molecular beacons for real-time pcr allelic discrimination of single nucleotide polymorphisms. Mol Cell Probes 2004, 18, 117-22.
2. Hahn, M., et al., Influence of fluorophor dye labels on the migration behavior of polymerase chain reaction–amplified short tandem repeats during denaturing capillary electrophoresis. Electrophoresis 2001, 22, 2691-700.